An experiment to transform dna found in the bacteria e coli

The plate was a lawn LB - Many colonies were found where the plate was streaked with the E.

An experiment to transform dna found in the bacteria e coli

All of the dishes had some similarities; all had agar in them which was food for the E coli. But their differences were what set the, apart. One was LB, which only contained food for the E coli.

This one had food for the bacteria and the antibiotic. This had food and the antibiotic as well. This had food, the antibiotic and sugar. On the LB dish we got a lot of growth, which was what we wanted because it shows that the E coli was viable.

This is good too because it tells us that the ampicillin an antibiotic did in fact work to slow down in this case, completely cease the growth of our bacteria.

I definitely accidently put 3x the amount of transformation fluid that I was supposed to, so that is why the value is skewed. However, this dish still showed us that the plasmid was successfully inserted since it fought against the antibiotic and grew anyway!

Also, this dish did not fluoresce, which tells us the plasmid is not enough on its own to glow. Now for the most exciting dish: Not only did this dish give us about 52 colonies, but these colonies fluoresce under an ultraviolet beam! This shows us that by the process of gene regulation, the arabinose sugar was able to turn on the GFP glowing gene to let our E coli shine like we always knew they could.

This plate was an excellent example of how the expressions of genes do depend of their environment. What effect does heat-shocking have? In this experiment, we heat-shocked our E.

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The E coli membrane pores opened up during the heat shock, so it could more readily accept the plasmid we inserted. What was our transformation efficiency? Transformation efficiency is a value which tells us how well we were able to insert the plasmid into the bacteria.

Our transformation efficiency number was Transformation efficiency tells us that the extent to which we genetically transformed E.

The lab concludes that a normal rage of efficiency should be around transformants per microgram. We were obviously slightly under that range. This could have been due to how strong our ampicillin pGLO was not that effective in being resistant to the ampicillin.

Another possibility could have been that we did not spread enough pGLO which contained the enzyme to resist ampicillin onto the dish.

An experiment to transform dna found in the bacteria e coli

What was the purpose of the antibiotic resistance? We had to use a plasmid that showed resistance to the bacteria-killing antibiotic ampicillin, in order for our E.

The bacteria at the end of our experiment could not only glow green under a UV light, but it was also able to resist a specific antibiotic ampicillin. Also, our experiment supports the notion that that the GFP gene is activated by the sugar arabinose, which is what lets jellyfish and our E coli fluoresce.transform a strain of E.

coli DH5a to be resistant to both ampicillin and kanamycin by creating recombinant pUC18 plasmid by restriction digestion and cloning day 2: . Transformation of the bacterium E. coli using a gene for Green Fluorescent Protein Background Bacteria and viruses can move DNA (or RNA) into an organism and cause of marker or reporter genes in molecular biology experiments.

3. Investigate how DNA .

Bacterial Transformation Lab: Manipulation of E. Coli. by Ben Chen on Prezi

Bacteria and viruses can move DNA (or RNA) into an organism and cause leslutinsduphoenix.com, lives in the human gut and is a relatively simple and well understood organism. Its genetic material consists mostly of one large circle of DNA million base pairs in length, with small bacteria and the decontamination necessary when the experiment is.

In science, it is always a mistake not to doubt when facts do not compel you to affirmPasteur. For the cost of Kyoto in just , we could once and for all solve the single biggest problem on earth.

Recombinant DNA technology - In vitro mutagenesis | leslutinsduphoenix.com

This presidential election has been notable in many regards, but perhaps most conspicuously in the preoccupation of the media with the health of the Democratic and Republican candidates. Transformation Background: Transformation is a process of transferring genetic information from one organism to another.

In bacteria, a small circular piece of DNA known as a plasmid (Table 1), transfers genetic information between bacteria, allowing these microbes to gain antibiotic resistance and adapt to new environments.

Activity 4: Transformation of E. coli using green fluorescent protein